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Projects

MCC Pilot Projects

A large portion of funds that have been received have been used to create, expand, and enhance shared resource facilities at the Moffitt Cancer Center. Shared Resources house specialized equipment and employ trained scientific staff in a specific scientific discipline. These facilities are shared by all investigators, minimizing the costs of equipment outlay for individual researchers and maximizing efficiency and reproducibility.

Once the ability to perform state-of-the-art genomics studies was ensured through the NFGC's funding of the shared resources, it became clear that a mechanism was needed to encourage the use of these facilities and to promote genomics research being conducted at Moffitt. This was necessary in order to fulfill one of the basic tenets of the establishment of the NFGC: to provide the framework for translational research that results in the rapid development of diagnostic tests and new drug therapies. It was decided that the best way to encourage translational research was to provide “seed” money to individual investigators to establish research projects for pilot research projects on the functional genomics of cancer, particularly as they related to molecular diagnostics and prognostics, predicting response to therapy, and development of new therapeutic modalities.

The call for project proposals was extended to all Moffitt faculty at the rank of Assistant Professor or above. A Working Group of ten Moffitt faculty with expertise in various aspects of genomics research was convened to evaluate the proposals. Proposals were selected based on three main criteria:

  1. Quality of science: Proposals were to be hypothesis-driven, and of a caliber that might eventually lead to the submission of grants for external funding. This will validate one of the primary objectives of the NFGC, to eventually become self-sustaining.
  2. Relevance to NFGC goals: Proposals that took advantage of the enhanced shared resources were given preference
  3. Translational implications: Research projects leading to clinical applications were given preference.

After the NFGC received its first year funds, 21 pilot projects of $25,000-$50,000 each were awarded.

The investigators and the titles of their projects are listed below:

  1. Deepak Agrawal, PhD
    “Identification of Tumor Cell and Microenvironment Interactions by Microarray Analysis”

  2. Scott Antonia, MD, PhD
    “Discovery of Gene Targets for Skin Cancer in Puerto Rico”

  3. Gerold Bepler, MD, PhD
    "Aberrations in Genomic DNA and Expression of DNA Damage Repair Genes”

    Click here for a summary of this project.

    Resulting publication:
    Zheng Z, Cantor A, Bepler G. A Global Genome Damage Score Predictive of Lung Cancer Patients Outcome. Oncogene. 2006 July 27; 25(32):4491-4. Epub 2006 Mar 6. PMID: 16518406

  4. Kapil Bhalla, MD
    “Identification of Genes that Regulate Growth Arrest and Apoptosis of Chronic Myeloid Leukemia Cells Following Treatment with Deacetylase Inhibitors and 5'-Aza-2'deoxycytidine”

    Resulting publications:

    Fiskus W, Pranpat M, Bali P, Balasis M, Kumaraswamy S, Boyapalle S, Rocha K, Wu J, Giles F, Manley PW, Atadja P, Bhalla K. Combined effects of novel tyrosine kinase inhibitor AMN107 and histone deacetylase inhibitor LBH589 against Bcr-Abl expressing human leukemia cells. Blood. 2006 Jul 15; 108(2):645-52. Epub 2006 Mar 14. PMID: 16537804

    George P, Bali P, Annavarapu S, Scuto A, Fiskus W, Guo F, Sigua C, Sondarva G, Moscinski L, Atadja P, Bhalla K. Combination of histone deacetylase inhibitor LBH589 and the hsp90 inhibitor 17-AAG is highly active against human CML-BC cells and AML cells with activating mutation of FLT-3. Blood. 2005 Feb 15; 105(4):1768-76. Epub 2004 Oct 28. PMID: 15514006

    Guo F, Sigua C, Bali P, George P, Fiskus W, Scuto A, Annavarapu S, Mouttaki A, Sondarva G, Wei S, Wu J, Djeu J, Bhalla K. Mechanistic role of heat shock protein 70 in Bcr-Abl-mediated resistance to apoptosis in human acute leukemia cells. Blood. 2005 Feb 1; 105(3): 1246-55. Epub 2004 Sept 23. PMID: 15388581

  5. Srikumar Chellappan, PhD
    “Gene Expression Patterns in Nicotine/NKK Induced Lung Cancers”

  6. Doug Cress, PhD
    “ Role of the E2F Family in Chemotherapy Induced Cell Death”

    Click here for a summary of this project.

    Resulting publications:

    Ma Y, Freeman SN, Cress WD. E2F4 Deficiency Promotes Drug Induced Apoptosis. Cancer Biol Ther. 2004 Dec; 3(12):1262-9 Epub 2004 Dec 14. PMID: 15611646

    Rodriguez JM, Glozak MA, Ma Y, Cress WD. Bok, Bcl-2 related ovarian killer, is cell cycle regulated and sensitizes to stress-induced apoptosis. J Biol Chem. 2006 Aug 11; 281(32):22729-35. Epub 2006 Jun 13. PMID: 16772296

  7. Adil Daud, MD
    “Prediction of Response to Chemotherapy by Gene Expression Profiling in Metastatic Melanoma”

    Resulting publication:
    Riker AI, Enkemann SA, Fodstad O, Liu S, Ren S, Morris C, Xi Y, Howell P, Metge B, Samant RS, Shevde LA, Li W, Eschrich S, Daud A, Ju J, Matta J. The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis. BMC Med Genomics. 2008 April 28; 1:13. PMID: 18442402

  8. Nils Diaz, MD
    “Molecular Signature Classification of Breast Carcinoma”

    Click here for a summary of this project.

  9. Mayer Fishman, MD
    “T-Lymphocyte mRNA Profile Marking Capacity for Anticancer Response”

  10. Eric Haura, MD
    “Role of STAT Signaling in Lung Cancer"

    Resulting publication:
    Dauer DJ, Ferraro B, Song L, Yu B, Mora L, Buettner R, Enkemann S, Jove R, Haura EB. Stat3 regulates genes common to both wound healing and cancer. Oncogene. 2005 May 12; 24(21):3397-408. PMID: 15735721

    Click here for a summary of this project.

  11. Lori Hazlehurst, PhD
    “Identification of Adhesion Mediated Genomic Signature in AML”

    Click here for a summary of this project.

  12. Richard Jove, PhD
    “Molecular Signature of Imatinib (Gleevec) Resistant and PD180970 Sensitive CML Cells”

  13. William Kerr, PhD
    “Microarray Identification of Genes Involved in Graft Versus Host Disease (GvHD)”

    Click here for a summary of this project.

  14. Johnathan Lancaster, MD, PhD
    “Identification of Discrete Genetic Pathways that Determine Survival from Ovarian Cancer Using Microarray Analysis”

    Resulting publications:

    Dressman HK, Berchuck A, Chan G, Zhai J, Bild A, Sayer R, Cragun J, Clarke J, Whitaker RS, Li L, Gray J, Marks J, Ginsburg JS, Potti A, West M, Nevins JR, Lancaster JM. An Integrated Genomic-Based Approach to Individualized Treatment of Patients With Advanced-Stage Ovarian Cancer. J Clin Oncol. 2007 Feb 10; 25(5): 517-525. PMID: 17290060

    Berchuk A, Iversen ES, Lancaster JM, Pittman J, Luo J, Lee P, Murphy S, Dressman HK, Febbo PG, West M, Nevins JR, Marks JR. Patterns of gene expression that characterize long-term survival in advanced stage serous ovarian cancers. Clin Cancer Res. 2005 May 15; 11(10):3686-96. PMID: 15897565

    Berchuck A, Iversen ES, Lancaster JM, Dressman HK, West M, Nevins JR, Marks JR. Prediction of optimal versus suboptimal cytoreduction of advanced-stage serous ovarian cancer with the use of microarrays. Am J Obstet Gynecol. 2004 Apr; 190(4):910-25. PMID: 15118612

  15. Subhra Mohapatra, PhD
    "Identification of Primary Cellular Targets of Purine Analogues in HTLV-1-infected Leukemic T Cell Lines by cDNA Microarray Analysis"

  16. Pamela Munster, MD
    “Potentiation of Topoisomerase Inhibitors by Histone Deacetylase Inhibitors”

    Resulting publications:

    Marchion DC, Bicaku E, Daud AI, Sullivan DM, Munster PN. In vivo synergy between topoisomerase II and histone deacetylase inhibitors: predictive correlates. Mol Cancer Ther. 2005 Dec; 4(12):1993-2000. PMID: 16373714

    Marchion DC, Bicaku E, Turner JG, Daud AI, Sullivan DM, Munster PN. Synergistic interaction between histone deacetylase and topoisomerase II inhibitors is mediated through topoisomerase IIbeta. Clin Cancer Res. 2005 Dec 1; 11(23):8467-75. PMID: 16322310

    Marchion, DC, Bicaku E, Daud AI, Sullivan DM, Munster PN. Valproic Acid Alters Chromatin Structure by Regulation of Chromatin Modulation Proteins. Cancer Research. 2005. May 1; 65(9):3815-22. PMID: 15867379

  17. Said Sebti, PhD
    “Identification and Characterization of Farnesylated and Geranylgeranylated Proteins from the Human Genome: A Prenylomic Project”

    Click here for a summary of this project.

  18. Edward Seto, PhD
    “Regulation of Gene Expression by Histone Deacetylase Enzymes”

  19. Daniel Sullivan, MD
    “Pharmacogenetic Prediction of Alkylating Agent Toxicity Prior to and During High-Dose Chemotherapy for Multiple Myeloma using Affymetrix High Density Single Nucleotide Polymorphism (SNP) GeneChip Arrays”

  20. Jie (Jerry) Wu, PhD
    “Gab1 Pleckstrin-Homology (PH) Domain as a Novel Molecular Target for Cancer Therapy”

  21. Timothy Yeatman, MD
    “Prediction of Response to Neoadjuvant Chemoradiotherapy by Gene Expression Profiling in Gastrosophageal Junction Tumors”

Page edited 7/7/09